After a penetrating lesion in the central nervous system, astrocytes enlarge, divide, and participate in creating an environment that adversely affects neuronal regeneration. We have recently proven that the neural cell adhesion molecule (N-CAM) partially inhibits the division of early postnatal rat astrocytes in vitro. Animals have been lesioned in the cerebral cortex, hippocampus, or striatum with a Hamilton syringe and needle at defined stereotaxic positions. On one side, the lesions had been concomitantly infused with N-CAM or with one of the N-CAM-related molecules. As a management, a peptide of the identical composition because the N-CAM decapeptide but of random sequence was infused on the contralateral facet of the brain. The inhibition was biggest within the cortical lesion sites (approximately 50%) and was less pronounced within the hippocampus (approximately 25%) and striatum (approximately 20%). Two weeks after the lesion, the cerebral cortical websites infused with N-CAM continued to exhibit a considerably smaller inhabitants of dividing astrocytes than the sites on the alternative side.
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- We have recently shown that the neural cell adhesion molecule (N-CAM) partially inhibits the division of early postnatal rat astrocytes in vitro.
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I need help in finishing these projects, currently. This is simple level and in case you are professional in c++, can be carried out in soon. Janeczko K. The proliferative response of astrocytes to injury in neonatal rat brain. A combined immunocytochemical and autoradiographic study.
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